Gregory H Underhill

Research Interests

• Liver tissue engineering, liver development, cancer microenvironments
• Tissue engineering, stem cell differentiation, microfabrication, biomaterials, high-throughput analysis

Primary Research Area

• Molecular, Cellular and Tissue Engineering

Research Areas

• Biomaterials
• Engineered tissue tolerance
• High-throughput analysis
• Liver development
• Liver-blood interactions
• Microfabrication
• Stem cell differentiation
• Tumor microenvironments

Selected Articles in Journals

• Jain I, Brougham-Cook A, Underhill GH. Effect of distinct ECM microenvironments on the genome-wide chromatin accessibility and gene expression responses of hepatic stellate cells. Acta Biomater. 2023 Sep 1; 167:278-292.
• Ryoo H, Underhill GH. Spatially Defined Cell-Secreted Protein Detection Using Granular Hydrogels: uGeLISA. ACS Biomater Sci Eng. 2023 May 8;9(5):2317-2328.
• Brougham-Cook A, Kimmel HRC, Monckton CP, Owen D, Khetani SR, Underhill GH. Engineered matrix microenvironments reveal the heterogeneity of liver sinusoidal endothelial cell phenotypic responses. APL Bioeng. 2022 Dec;6(4):046102.
• Monckton CP, Brougham-Cook A, Underhill GH, Khetani SR. Modulation of human iPSC-derived hepatocyte phenotype via extracellular matrix microarrays. Acta Biomater. 2022 Nov;153:216-230
• Jain I, Berg IC, Acharya A, Blaauw M, Gosstola N, Perez-Pinera P, Underhill GH. Delineating cooperative effects of Notch and biomechanical signals on patterned liver differentiation. Commun Biol. 2022 Oct 7;5(1):1073.
• Zambuto SG, Jain I, Clancy KBH, Underhill GH, Harley BAC. Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels. ACS Biomater Sci Eng. 2022 Sep 12;8(9):3819-3830.
• Berent ZT, Jain I, Underhill GH, Wagoner Johnson AJ. Simulated confluence on micropatterned substrates correlates responses regulating cellular differentiation. Biotechnol Bioeng. 2022 Jun;119(6):1641-1659.
• Brougham-Cook A, Jain I, Kukla DA, Masood F, Kimmel H, Ryoo H, Khetani SR, Underhill GH. High throughput interrogation of human liver stellate cells reveals microenvironmental regulation of phenotype. Acta Biomater. 2022 Jan 15;138:240-253
• Monckton CP, Brougham-Cook A, Kaylan KB, Underhill GH, Khetani SR. Elucidating Extracellular Matrix and Stiffness Control of Primary Human Hepatocyte Phenotype Via Cell Microarrays. Adv Mater Interfaces. 2021 Nov 23;8(22)
• Berg IC, Mohagheghian E, Habing K, Wang N, Underhill GH. Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D. Adv Healthc Mater. 2021 Jun;10(12):e2100223
• Gentile SD, Kourouklis AP, Ryoo H, Underhill GH. Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids. Front Bioeng Biotechnol. 2020;8:792.
• Underhill GH, Khetani SR. Emerging trends in modeling human liver disease in vitro. APL Bioeng. 2019 Dec;3(4):040902.
• Berg IC, Underhill GH. High Throughput Traction Force Microscopy for Multicellular Islands on Combinatorial Microarrays. Bio Protoc. 2019 Nov 5;9(21).
• Kaylan KB, Berg IC, Biehl MJ, Brougham-Cook A, Jain I, Jamil SM, Sargeant LH, Cornell NJ, Raetzman LT, Underhill GH. Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling. Elife. 2018 Dec 27;7.
• Underhill GH, Khetani SR. Advances in Engineered Human Liver Platforms for Drug Metabolism Studies. Drug Metab Dispos. 2018 Nov;46(11):1626-1637.