BIOE: Sepideh Dolatshahi - "Systems Analysis of Glycosylated Antibodies: Regulation, Engineering and Functions"
(sign-up)Sepideh Dolatshahi, Postdoctoral Associate, Department of Biological Engineering, Massachusetts Institute of Technology
2233 Everitt Laboratory, 1406 W Green St, Urbana, IL
|Event Contact:||Lisa Leininger
Department of Bioengineering
*** Please note the location change to 2233 Everitt Laboratory and time change to 11:30 a.m. ***
“Systems Analysis of Glycosylated Antibodies: Regulation, Engineering and Functions”
N-linked glycosylation is a post-translational modification that affects potency, safety, immunogenicity, immune effector functions, and clearance of several therapeutic proteins including antibodies. These modifications are shaped by cellular and enzyme-mediated processes, regulated at both transcriptional and metabolic levels. However, their dynamics remains insufficiently understood due to the lack of integrative and quantitative analyses of contributing factors. I will describe a systems biology approach that includes multi-dimensional omics analyses and mathematical modeling to develop a mechanistic understanding of N-glycosylation of antibodies produced during fed-batch cultures of producer cells. Together, these insights provide tools for precision control of N-glycosylation. The functional role of glycosylation will then be probed in a representative study, where selective transfer of antibodies with di-galactosylated Fc-glycan profiles across the placenta from mothers to newborns is observed. This study provides critical insights for the design of next generation maternal vaccines engineered to elicit antibodies that will effectively enhance immunity in neonates.
About the Speaker:
Dr. Sepideh Dolatshahi is a Postdoctoral Fellow, working with Prof. Doug Lauffenburger in the Department of Biological Engineering at MIT. She received her Ph.D. in 2015 from Georgia Institute of Technology under the supervision of Prof. Eberhard Voit, where she developed advanced methodologies for the modeling of metabolic pathway systems based on time series data. Her primary research interests are systems biology of immune regulation and immunotherapies, and inter-species translation of cellular processes in animals to those in humans.
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